g rabbit anti human collagen i α1 (Novus Biologicals)
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Novus Biologicals
g rabbit anti human collagen i α1
G Rabbit Anti Human Collagen I α1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+i/Collagen+I+Antibody/pmc13652712-112-82-88
Average 96 stars, based on 269 article reviews
G Rabbit Anti Human Collagen I α1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+i/Collagen+I+Antibody/pmc13652712-112-82-88
Average 96 stars, based on 269 article reviews
g rabbit anti human collagen i α1 - by Bioz Stars,
2026-10
96/100 stars
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Staining:Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling. Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Immunohistochemical staining:Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling. Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Incubation:Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Article Title: MicroRNA-130 as a critical modulator of cardiac remodeling: interplay between autophagic flux and ferroptotic pathways in acute myocardial infarction. Article Snippet: Following RNA isolation from H9C2 cells via the Tiangen RNAsimple Total RNA Kit (Beijing, China), nucleic acid quantification and quality assessment were conducted spectrophotometrically (NanoDrop, Thermo Fisher), after which reverse transcription utilizing Thermo Fisher's RevertAid cDNA Synthesis Kit was Jo urn al Pr e-p roo f implemented with customized stem-loop primers (miR-130-5p: F 5′-AAAGGCACCTGGGAAGTCATCG-3′, R 5′-CAGCTCACAACTGAGGCATGTC-3′; U6 snRNA: F 5′-CTCGCTTCGGCAGCACA-3′, R 5′-AACGCTTCACGAATTTGCGT-3′) for cDNA generation, succeeded by quantitative amplification on Roche's LightCycler480 platform employing Tiangen SuperReal PreMix Plus under optimized thermal parameters (95°C/30s initiation; 45 cycles of 95°C/5s denaturation and 60°C/30s annealing/extension), with subsequent 2−ΔΔCt-based [26] normalization against U6 endogenous controls for relative miRNA expression quantification. .. Protein lysates from H9C2 cardiomyocytes were prepared using NCM biotech (NCM biotech, Suzhou, China) RIPA buffer, quantified via BCA assay (NCM biotech), resolved electrophoretically (20 μg, 10% SDS-PAGE), and transferred to nitrocellulose membranes (Millipore, USA), which were subsequently blocked with 3% BSA/TBST and probed overnight at 4°C with primary antibodies targeting α-SMA (1:1000, ab7817) and GPX4 (1:1000, ab125066) form Abcam, Article Title: Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix Remodeling. Article Snippet: .. The sections were then sequentially stained with Weigert’s iron hematoxylin for 10 min, Biebrich scarlet acid fuchsin solution for 10 min, phosphomolybdic/phosphotungstic acid for 10 min, aniline blue for 10 min, and 1% acetic acid for 5 min. For Immunohistochemical staining, unstained sections were pretreated in a microwave, blocked with serum, and incubated with primary antibodies, including F4/80 (CI:A3-1, #NB600-404; Novus Biologicals, Centennial, CO, USA), TGF-β1 (TB21, #GTX21279; GeneTex, Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and Article Title: Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGel. Article Snippet: Determination of Decellularization Efficiency—Immunofluorescence Assay: Transversal slices of native and decellularized tissues were embedded in OCT, sectioned by a cryostat, and permeabilized in 0.3% TRITON X-100 (Sigma-Aldrich) for 10 min at room temperature (RT). .. The samples were first incubated in 5% Bovine Serum Albumin (BSA) blocking solution for 30 min to saturate the nonspecific sites, and then overnight at 4 °C with anti-Sarcomeric Alpha Actinin (αSARC; 1:100, Bioss-Antibodies, bs-10367R), cardiac Troponin T (cTnT; 1:100, Abcam, ab8295), Article Title: HIV-1 Amplifies IL-8 Response Of Human Stellate Cells To Gram-Positive Microbial Products Via H4K5 Histone Acetylation Article Snippet: .. Membranes were washed 3 times with blotting buffer, incubated at 4 0 C overnight with primary antibody (1:1000) containing 5% lowfat powdered milk, and incubated with secondary antibody (1:1000) at room temperature for 60 min. Target proteins were developed with TMB 1-component membrane solution (SeraCare Life Sciences Inc., Milford, MA). α-SMA (NOVUS, Cat No. NBP 1-45433 and NB100-56565), Article Title: Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGel Article Snippet: Transversal slices of native and decellularized tissues were embedded in OCT, sectioned by a cryostat, and permeabilized in 0.3% TRITON X‐100 (Sigma‐Aldrich) for 10 min at room temperature (RT). .. The samples were first incubated in 5% Bovine Serum Albumin (BSA) blocking solution for 30 min to saturate the nonspecific sites, and then overnight at 4 °C with anti‐Sarcomeric Alpha Actinin (αSARC; 1:100, Bioss‐Antibodies, bs‐10367R), cardiac Troponin T (cTnT; 1:100, Abcam, ab8295), BIA-KA:Article Title: MicroRNA-130 as a critical modulator of cardiac remodeling: interplay between autophagic flux and ferroptotic pathways in acute myocardial infarction. Article Snippet: Following RNA isolation from H9C2 cells via the Tiangen RNAsimple Total RNA Kit (Beijing, China), nucleic acid quantification and quality assessment were conducted spectrophotometrically (NanoDrop, Thermo Fisher), after which reverse transcription utilizing Thermo Fisher's RevertAid cDNA Synthesis Kit was Jo urn al Pr e-p roo f implemented with customized stem-loop primers (miR-130-5p: F 5′-AAAGGCACCTGGGAAGTCATCG-3′, R 5′-CAGCTCACAACTGAGGCATGTC-3′; U6 snRNA: F 5′-CTCGCTTCGGCAGCACA-3′, R 5′-AACGCTTCACGAATTTGCGT-3′) for cDNA generation, succeeded by quantitative amplification on Roche's LightCycler480 platform employing Tiangen SuperReal PreMix Plus under optimized thermal parameters (95°C/30s initiation; 45 cycles of 95°C/5s denaturation and 60°C/30s annealing/extension), with subsequent 2−ΔΔCt-based [26] normalization against U6 endogenous controls for relative miRNA expression quantification. .. Protein lysates from H9C2 cardiomyocytes were prepared using NCM biotech (NCM biotech, Suzhou, China) RIPA buffer, quantified via BCA assay (NCM biotech), resolved electrophoretically (20 μg, 10% SDS-PAGE), and transferred to nitrocellulose membranes (Millipore, USA), which were subsequently blocked with 3% BSA/TBST and probed overnight at 4°C with primary antibodies targeting α-SMA (1:1000, ab7817) and GPX4 (1:1000, ab125066) form Abcam, Blocking Assay:Article Title: Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGel. Article Snippet: Determination of Decellularization Efficiency—Immunofluorescence Assay: Transversal slices of native and decellularized tissues were embedded in OCT, sectioned by a cryostat, and permeabilized in 0.3% TRITON X-100 (Sigma-Aldrich) for 10 min at room temperature (RT). .. The samples were first incubated in 5% Bovine Serum Albumin (BSA) blocking solution for 30 min to saturate the nonspecific sites, and then overnight at 4 °C with anti-Sarcomeric Alpha Actinin (αSARC; 1:100, Bioss-Antibodies, bs-10367R), cardiac Troponin T (cTnT; 1:100, Abcam, ab8295), Article Title: Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGel Article Snippet: Transversal slices of native and decellularized tissues were embedded in OCT, sectioned by a cryostat, and permeabilized in 0.3% TRITON X‐100 (Sigma‐Aldrich) for 10 min at room temperature (RT). .. The samples were first incubated in 5% Bovine Serum Albumin (BSA) blocking solution for 30 min to saturate the nonspecific sites, and then overnight at 4 °C with anti‐Sarcomeric Alpha Actinin (αSARC; 1:100, Bioss‐Antibodies, bs‐10367R), cardiac Troponin T (cTnT; 1:100, Abcam, ab8295), Membrane:Article Title: ChREBP drives fibroblast proliferation and promotes pulmonary fibrosis development. Article Snippet: Objective: The study aimed to investigate the role of carbohydrate-responsive element-binding protein (ChREBP) in the pathogenesis of pulmonary fibrosis (PF) by assessing its impact on fibrotic protein expression, fibroblast proliferation, and apoptosis in lung tissues.. Method: The PF model was established using bleomycin, and pathological changes in lung tissues were assessed through histopathological analysis.. Expression levels of inflammatory markers and fibrotic proteins, including ChREBP, were measured using Western blot and ELISA. Article Title: HIV-1 Amplifies IL-8 Response Of Human Stellate Cells To Gram-Positive Microbial Products Via H4K5 Histone Acetylation Article Snippet: .. Membranes were washed 3 times with blotting buffer, incubated at 4 0 C overnight with primary antibody (1:1000) containing 5% lowfat powdered milk, and incubated with secondary antibody (1:1000) at room temperature for 60 min. Target proteins were developed with TMB 1-component membrane solution (SeraCare Life Sciences Inc., Milford, MA). α-SMA (NOVUS, Cat No. NBP 1-45433 and NB100-56565), other:Article Title: Compounds and methods for treating fibrotic pathologies Article Snippet: Wells were washed with TBS-Tween before 45 minute incubation with IR-dye-conjugated secondary antibody (Li-Cor #926-32211) diluted 1:400. |
